Rate of penetration of alcohols

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Rate of penetration of fixatives

Phospholipid component volumes. As a purely structural effect, it is clear that the membrane thus becomes more permeable to small molecules due to its increased area per lipid. Support Center Support Center. Interestingly, ethanol and methanol have almost the same effect on the area per lipid. It is found that ethanol has a stronger effect on the structural properties of the membranes. Unfortunately, a direct comparison is not meaningful, since that study was done in the gel phase using multilamellar vesicles, as compared to the fluidlike phase and planar bilayer system studied here. They were needed for a detailed comparison with experiments and other simulations 12 , Dark solid line means that the alcohol molecule is hydrogen-bonded to a lipid and the dotted line means it is not. Here, we have characterized simple membrane-alcohol systems. The above observation is also able to explain why no hydrogen bonds are formed between methanol and the lipids. This was observed in a recent experiment 49 where the potassium channel KcsA was observed to dissociate due to the changes in lateral membrane pressure induced by small alcohols. In our simulations, this is demonstrated by the decrease of the order parameter for the lipids that are bound to ethanols Fig.

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made concerning the actual rates of penetration of fixatives are extremely used in fixing fluids, namely, acetic acid, mercuric chloride, ethyl alcohol, osmium.

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Description: Let us first discuss the area per lipid and bilayer thickness. About the capillarity constants of organic materials in watery solutions. Physically, the surface tension is modified by the presence of ethanol. After 20 ns, the samples had equilibrated, and the remaining 30 ns were used for data collection. The force-field parameters for bonded and nonbonded interaction were taken from Berger et al. Phospholipid bilayers can be considered as a first approximation to understand the behavior of cell membranes under the influence of alcohol, and much information can be extracted from such systems. Abstract Extensive microscopic molecular dynamics simulations have been performed to study the effects of short-chain alcohols, methanol and ethanol, on two different fully hydrated lipid bilayer systems POPC and DPPC in the fluid phase at K. The results presented in Fig. Additional information can be gained by considering separately the two charged groups in the lipid headgroups, namely the phosphate P and the choline N group see Fig. Hydrogen-bonding analysis offers a well-defined criterion to decide whether a given lipid is interacting strongly with an alcohol molecule.
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